Nitrile hydratase (NHase) is a promising biocatalyst for the industrial synthesis of high value amides, yet its practical application is often limited by conformational instability and activity loss under high product concentrations. In this study, we employed a rational engineering strategy guided by molecular dynamics simulations in high amide concentrations to elucidate the structural dynamics of NHase acrylamide/water mixe...

This paper addresses the bidirectional multi-chamber lock scheduling problem by formulating a multi-objective mixed-integer linear programming (MILP) model that simultaneously minimizes average ship waiting time and maximizes chamber utilization. A tailored adaptive large neighborhood search (ALNS) algorithm is developed specifically based on the principle of the destruction and reconstruction of solutions. The algorithm effic...