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Evaluation of the APTIMA Combo 2™ kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semenNARA Subscribed
Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infe...
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