Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of...

Trophic interactions are important determinants of the structure and functioning of ecosystems. Because the metabolism and consumption rates of ectotherms increase sharply with temperature, there are major concerns that global warming will increase the strength of trophic interactions, destabilizing food webs, and altering ecosystem structure and function. We used geothermally warmed streams that span an 11°C temperature gradi...

Gross primary production ( GPP ) is the largest flux in the carbon cycle, yet its response to global warming is highly uncertain. The temperature dependence of GPP is directly linked to photosynthetic physiology, but the response of GPP to warming over longer timescales could also be shaped by ecological and evolutionary processes that drive variation in community structure and functional trait distributions. Here, we show tha...

Marine microalgae ( Emiliania huxleyi ) were grown in seawater enriched only with nitrogen and phosphorus, without control of free metal concentrations using synthetic chelators. Complexing ligands and thiol compounds were determined by cathodic stripping volt–ammetry. Copper was added to these cultures, and ligands were produced in response to the copper addition. Parallel measurements of thiols showed that glutathione and ot...