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Establishment and Application of a Triple TaqMan qPCR Detection Method for IMNV, DIV1, and CMNVNARA Subscribed
The escalating incidence of emerging pathogens and their propensity for co‐infection pose a severe threat to the sustainable intensification of Penaeus vannamei aquaculture. To address this challenge, a novel triplex TaqMan quantitative PCR (qPCR) assay was developed for the simultaneous detection of three high‐risk agents: Infectious Myonecrosis Virus (IMNV), Decapod Iridescent Virus 1 (DIV1), and Covert Mortality Nodavirus (...
Subsea tunnels constructed by the drill-and-blast method are increasingly required in modern infrastructure and are often exposed to high groundwater pressure and fractured rock conditions. In such environments, external water pressure acting on initial support strongly affects tunnel stability, durability, and construction safety. Because the initial support is temporary, discontinuous, and prone to cracking, evaluation of it...