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Molecular characterization of extended-spectrum β-lactamases-producing Escherichia coli isolated from patients with bacteremiaNARA Subscribed
Bacteremia is a major health problem that affects millions of people worldwide. E. coli is one of the main pathogens associated with bacteremia and its resistance to the antimicrobial drugs, including β-lactams, has significantly increased in recent years. In view of the above, our objective was to determine the antimicrobial resistance profile of 60 E. coli isolates obtained from patients with bacteremia, as well as to charac...
Atta robusta is a narrow endemic leaf-cutting ant restricted to restinga habitats along Brazil’s southeastern coast. Despite its conservation status, little is known about its evolutionary history and genetic structure. Here, we investigate patterns of genetic variation in A. robusta using mitochondrial (COI–IGS–tRNALeu–COII) and nuclear (EF1alpha copy one) DNA markers sampled across its known range. We assess genetic diversit...
Promoter Engineering of the Surfactin Operon Enhances Surfactin Production in the Environmental Strain Bacillus subtilis RI4914NARA Subscribed
This study aimed to enhance surfactin production in Bacillus subtilis by replacing the native promoter of the surfactin synthase operon (P srfA ) with the strong IPTG-inducible promoter Pgrac to address challenges in large-scale biosurfactant production. The engineered strain was developed through homologous recombination, creating an integration cassette fused via overlap extension PCR and transformed into B. subtilis RI4914....
Aim We investigate the distribution of dispersal modes — endozoochory (via animal ingestion), synzoochory (via animal seed accumulation) and anemochory (via wind)—and seed mass of woody plants across Brazilian ecosystems, testing whether their relative abundance in local plant communities could be predicted by climate, edaphic factors and dispersal agents (e.g., the main vectors moving seeds). We expected climate to be the mai...
Potato yield is highly dependent on phosphorus (P) availability. Although high rates of P fertilizers are commonly applied to potatoes even in high P test soils, the efficiency of these practices is questionable. Foliar P fertilization, particularly during the tuber bulking stage, has emerged as a potential complementary strategy. This study aimed to evaluate whether adjusting the conventional P application rate at planting an...
Deforestation impacts on environmental conditions of headwater streams and Ephemeroptera (Insecta) diversity in Southwestern AmazoniaNARA Subscribed
The Amazon, home to the planet’s greatest biodiversity, faces increasingly intense and frequent degradation due to land-use changes and land conversion. These actions, although dominant in terrestrial systems, have a severe impact on aquatic ecosystems, which are closely dependent on surrounding landscapes, particularly riparian vegetation. Given this scenario, this study evaluated the influence of forest loss on environmental...
Unravelling the molecular mechanisms of vegetative-to-reproductive transition in Cynara cardunculus by RNA-Seq analysisNARA Subscribed
Cynara cardunculus inflorescence plays a significant role in cheese manufacturing and human consumption due to the milk-clotting capacity and the production of edible immature capitula. Consequently, it is crucial to understand the complex cellular and molecular processes involved in the development of cardoon flowers. The current study used western wild cardoon in two developmental stages, vegetative and reproductive, to unde...
Detection of physiological changes in knee cartilage using parametric T2 relaxation maps estimated with a dictionary methodNARA Subscribed
Purpose To investigate half-marathon running and recovery effects on knee articular cartilage (KAC) health in athletes, using dictionary-matching T 2 maps, to detect changes and recovery patterns in KAC. Methods Eleven asymptomatic volunteers (4 females, 7 males; mean age 40 ± 5 years, mean BMI 22.7 ± 1.4 kg/m 2 ) completed a fixed-pace half-marathonwere studied. All were right-knee dominant and engaged in regular running. Sag...
Understanding how small populations cope with loss of genetic diversity and deleterious variation is crucial to address the current biodiversity crisis. Insular populations are particularly interesting as they have often persisted at lower population sizes and higher inbreeding than their mainland counterparts. While the genome‐wide consequences of inbreeding in threatened insular species have received some attention, comparat...