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Urban wastewater systems represent important interfaces between human activity and the environmental occurrence of antimicrobial resistance (AMR) markers. We assessed the temporal dynamics of intI1 , ermB , and the 16 S rRNA gene by quantitative PCR across three wastewater systems (EPC, CJC, and JW) in Fortaleza, Brazil, from November 2021 to November 2023. Bacterial communities were additionally characterized by 16 S rRNA gen...
Promoter Engineering of the Surfactin Operon Enhances Surfactin Production in the Environmental Strain Bacillus subtilis RI4914NARA Subscribed
This study aimed to enhance surfactin production in Bacillus subtilis by replacing the native promoter of the surfactin synthase operon (P srfA ) with the strong IPTG-inducible promoter Pgrac to address challenges in large-scale biosurfactant production. The engineered strain was developed through homologous recombination, creating an integration cassette fused via overlap extension PCR and transformed into B. subtilis RI4914....
The Role of Gene Flow in the Diversification of the Monkey Treefrog Complex Across the South American Dry DiagonalNARA Subscribed
Understanding Neotropical megadiversity remains challenging due to fundamental taxonomic issues, including identifying and describing cryptic species and their distribution, and the limited knowledge of key factors driving biological diversification. Such challenges are especially prominent in diverse clades with high levels of cryptic species, such as many Neotropical frogs. Herein, we conduct a mitochondrial phylogenetic stu...
Aim We investigate the distribution of dispersal modes — endozoochory (via animal ingestion), synzoochory (via animal seed accumulation) and anemochory (via wind)—and seed mass of woody plants across Brazilian ecosystems, testing whether their relative abundance in local plant communities could be predicted by climate, edaphic factors and dispersal agents (e.g., the main vectors moving seeds). We expected climate to be the mai...
American Useful Plants in Post-Medieval Europe: Integrating Archaeobotanical and Historical EvidenceNARA Subscribed
The arrival of Europeans in the Americas in 1492 initiated new transatlantic connections that, through colonial expansion and trade networks, brought useful plants to Europe. This study investigates archaeobotanical and historical evidence for the introduction of American plants to Europe from the late fifteenth to the twentieth century. We compiled 183 archaeobotanical records from 14 countries, representing 24 taxa from 11 b...
Due to its high concentration of growth factors, platelet-rich plasma (PRP) has become the subject of much research, either as an isolated or adjuvant treatment for wound repair. The fractal dimension has been used as an innovative method for the characterization and quantification of tissue structural alterations without any interference from the evaluator. The objective of this study was characterizing collagen types I and I...
The restoration of biodiversity and functional tropical forests is critical to mitigating global biodiversity losses. Aboveground, increasing the connectivity of regenerating forest fragments facilitates the recolonization of tropical forest biodiversity. However, restoring functional ecosystems also requires the recovery of decomposition processes as these are essential in shaping aboveground biodiversity. Therefore, we inves...
Quantifying animal movements is necessary for answering a wide array of research questions in ecology and conservation biology. Consequently, ecologists have made considerable efforts to identify the best way to estimate an animal's home range, and many methods of estimating home ranges have arisen over the past half a century. Most of these methods fall into two distinct categories of estimators that have only recently been d...