Background Mycobacterium bovis BCG is widely used as a vaccine vector due to its safety, genetic stability, and immunomodulatory properties. However, current expression systems for BCG transformation rely on antibiotic resistance genes, raising biosafety concerns. Therefore, alternative strategies—such as the use of auxotrophic strains—are highly desirable. Our group previously employed BioBrick™ technology to construct the pU...

iNaturalist has been establishing itself as a bridge between researchers and the public, providing relevant data for scientific studies while making science more accessible beyond academia. Horseflies can serve as a useful model group when using the platform to expand distribution records, due to the relative ease of identifying members of the family from photographs. The study was organized into four stages: (i) defining the...

Research on vegetative propagation in the genus Khaya remains limited and has focused mainly on juvenile trees, which restricts the selection of plus trees previously tested for growth potential and economic value. This study aimed to evaluate the vegetative propagation of three African mahogany species by inducing epicormic shoots in adult trees, and to investigate evidence of rejuvenation/reinvigoration during adventitious r...

Purpose Interoperability among Life Cycle Inventory (LCI) formats remains a persistent challenge, even for widely used standards such as EcoSpold 2 and ILCD, limiting data exchange and reuse. Converters are commonly employed to bridge these gaps, yet existing tools are mostly commercial, tied to specific software ecosystems and lack robust open-source alternatives. To address this limitation, we introduce Lavoisier, an open-so...

This study investigates the performance of a sequencing batch electro-membrane bioreactor (SB-EMBR) operated under low electric charge loading (39.9 mAh L⁻ 1 ), focusing on the effects of aeration intensity on treatment performance, biomass activity, and membrane fouling. The reactor was operated at a current density of 10 A m −2 and three specific aeration demand levels (SADₘ = 0.48, 0.24, and 0.12 m 3 m⁻ 2 h⁻ 1 ). Organic ma...

Wildland-Urban Interface (WUI) areas are critical zones where human settlements and wildland fuels intersect, resulting in heightened wildfire risk and increased potential for losses. This study presents a two‑phase review of literature where artificial intelligence (AI) is applied in wildfire and WUI studies, combining bibliometric mapping for wildfires and a thematic content analysis for WUI. In Phase One, over 3500 publicat...

Geological faults are key discontinuities in sedimentary basins and strongly influence the hydraulic and mechanical behavior of hydrocarbon reservoirs. Fault zones typically contain a low-permeability core and a more permeable damage zone, generating sharp contrasts in petrophysical and mechanical properties. Stress variations from production, injection, or geological storage may induce fault reactivation, dilatancy, and perme...

Maintaining the nutritional quality of stored concentrates is crucial for the health and productivity of dairy herds, particularly in tropical climates where high temperatures can accelerate feed degradation. This study aimed to monitor the effects of temperature and humidity variations on the quality of pelleted concentrate feed in steel silos under real-world Brazilian farm conditions during 28-day storage. Three 4-ton steel...

In this paper, how changes to environmental aspects considered in strategic environmental assessment (SEA) can affect human health is explored, based on a comprehensive and systematic literature review. Whilst health is a key aspect for consideration in SEA, how it should be approached has remained subject to discussion. There is agreement, though, that at a minimum, health impacts from bio-physical environmental changes need...