Spring viremia of carp virus (SVCV), that can infect cyprinoid and koi causing an acute hemorrhagic symptom, with a feature of widespread epidemic, which poses a severe threat to the global aquaculture industry. Given the absence of specific effective medicine or vaccines, the early, accurate diagnosis is critical in the disease prevention. Many SVCV detection methods have been established, but most require specialized equipme...

Grass carp hemorrhagic disease, mainly caused by grass carp reovirus (GCRV), is a major threat to grass carp aquaculture, and vaccination is considered a sustainable strategy for disease prevention and control. However, the protective efficacy of GCRV vaccines and the factors influencing it have not been systematically synthesised. In this study, literature on GCRV vaccines was retrieved from CNKI, ScienceDirect, and Web of Sc...

Quasispecies represent a general evolutionary model for most RNA viruses and other viruses with high mutation rates. However, quasispecies have not been previously reported in the grass carp reovirus (GCRV), which has caused severe hemorrhagic disease in grass carp in China for several decades. This study revealed the presence of multiple co‐existing mutant sequences within individual viral strains, indicative of a quasispecie...

A spleen‐derived cell line was established from the spleen of hybrid snakehead (♀ Channa argus × ♂ Channa maculata ) (abbreviated as CAMsp ), a species of considerable economic importance in China's freshwater aquaculture, which is severely impacted by the hybrid snakehead rhabdovirus ( HSHRV ), largemouth bass ranavirus ( LMBV ), and infectious spleen and kidney necrosis virus ( ISKNV ). The establishment of a stable in vitro...

Largemouth bass ( Micropterus salmoides ), a freshwater fish species, is cultivated widely across China. The industry has been greatly affected by various viral diseases. We generated a new immortal cell line from the fin of M. salmoides (MSF). It has been successfully maintained in continuous culture for over 80 passages. Cells multiply well at 28°C using Medium 199 or Leibovitz's L‐15 supplemented with 10%–20% fetal bovine s...

DNA methylation is a critical mechanism for regulating gene expression in bacteria and plays an essential role in bacterial pathogenesis. A mutant, WC1535ΔhsdM, lacking hsdM encoding a DNA methyltransferase was constructed using homologous recombination technology. The growth, hemolytic activity, and capsule formation of the mutant were analyzed. The dynamic distribution of the wild-type (WT) and mutant strains in tilapia tiss...

In this issue, we established rapid, cost‐effective, and simple detection methods including recombines polymerase amplification with lateral flow dipstick (RPA‐LFD) and real‐time RPA for cyprinid herpesvirus 3(CyHV‐3), and evaluated their sensitivity, specificity, and applicability, the real‐time RPA method could achieve sensitive diagnosis of CyHV‐3 within 1.3 copies per reaction, respectively. The real‐time RPA method is 10‐...