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Plant Cell, Tissue and Organ Culture (PCTOC) · 2022 · Vol. 151 · Issue 1 · Springer
Bottle gourd [ Lagenaria siceraria (Mol.) Standl.] is one of the most common horticultural and medicinal crops. Genetic transformation is a rate-limiting step in unraveling the function of genes and molecular breeding in this non-model crop. In this study, we established an optimized protocol for transformation using a seed germination pouch as a growth carrier and developed bottle gourd composite plants. Overground explants w...