Summary In many eukaryotes, transcription factor MCM1 gene plays crucial roles in regulating mating processes and pathogenesis by interacting with other co‐factors. However, little is known about the role of MCM1 in rust fungi. Here, we identified two MCM1 orthologs, PstMCM1‐1 and PstMCM1‐2 , in the stripe rust pathogen Puccinia striiformis f. sp. tritici ( Pst ). Sequence analysis indicated that both PstMCM1‐1 and PstMCM1‐2 c...