Neuropeptide FF (NPFF) belongs to the RF-amide peptide family and is homologous to gonadotropin-inhibitory hormone (GnIH). The NPFF precursor encodes two mature peptides, NPFF and NPAF (neuropeptide AF). Both peptides share the conserved C-terminal PQRFa motif. However, there is very limited information available on receptor cross-reactivity for NPFF and GnIH peptides in teleosts. As a first step, we cloned two cognate recepto...