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Development of a Colourimetric Loop‐Mediated Isothermal Amplification Assay for Rapid Detection of Streptococcus dysgalactiaeNARA Subscribed
Streptococcus dysgalactiae is an emerging fish pathogen that causes streptococcosis, resulting in significant economic losses in aquaculture. Conventional detection methods, such as polymerase chain reaction assays, rely on sophisticated, expensive instrumentation, which limits their applicability in resource‐limited or field settings. In this study, we developed a rapid, user‐friendly and closed‐tube colourimetric loop‐mediat...
Development of a Visual Loop‐Mediated Isothermal Amplification Assay for Rapid Detection of Morganella morganiiNARA Subscribed
Morganella morganii is a Gram‐negative bacterium associated with foodborne diseases and histamine toxicity in fish, posing significant public health concerns. Traditional detection methods, such as polymerase chain reaction (PCR), often require costly equipment, restricting their applicability in resource‐limited environments. In this study, we present the development of a cost‐effective and user‐friendly colorimetric loop‐med...
Rapid Detection of Aeromonas veronii Using GGDEF Gene‐Based Colorimetric Loop‐Mediated Isothermal AmplificationNARA Subscribed
Aeromonas veronii has emerged as an important fish pathogen that poses threats to the aquaculture industry worldwide, and rapid detection is essential to minimise negative economic impact. In this study, a colorimetric loop‐mediated isothermal amplification (LAMP) assay was developed to rapidly detect A. veronii , targeting a gene encoding GGDEF domain‐containing diguanylate cyclase. The entire LAMP reaction could be completed...
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