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Shrimps regulate osmotic pressure by altering the amounts of free amino acids in their bodies. While changes in alanine (Ala) content are particularly significant, the molecular mechanisms involved in controlling Ala levels remain largely unknown. In the present study, we investigated alanine-glyoxylate aminotransferase 1 (AGT1), an l -Ala-degrading enzyme, using the kuruma shrimp Penaeus japonicus as a model to elucidate the...
Recent studies have revealed that stony corals create their extracellular skeletons via biologically controlled calcification, in which amorphous calcium carbonate (ACC), regarded as precursors of aragonite crystals, have been observed at nanoscale using electron microscopy. However, the exact mechanism by which ACC is generated, and how it contributes to skeletal growth in coral calcifying tissue, remains enigmatic. The septa...
Pilot study of a comprehensive resource estimation method from environmental DNA using universal D-loop amplification primersNARA Subscribed
Many studies have investigated the ability of environmental DNA (eDNA) to identify the species. However, when individual species are to be identified, accurate estimation of their abundance using traditional eDNA analyses is still difficult. We previously developed a novel analytical method called HaCeD-Seq (haplotype count from eDNA by sequencing), which focuses on the mitochondrial D-loop sequence for eels and tuna. In this...
The present study was undertaken to determine the amounts of tetrodotoxin (TTX) and its analogs (TTXs) in various tissues of toxin-bearing pufferfish (Canthigaster revulata and Takifugu flavipterus) and newt (Cynops pyrrhogaster) using specific polyclonal antibodies against TTXs, and to compare the obtained results with those mainly determined by high-performance liquid chromatography with fluorescence detection (HPLC-FLD). Th...
PIWI proteins and PIWI-interacting RNAs (piRNAs) suppress transposon activity in animals, thus safeguarding the genome from detrimental insertion mutagenesis. Recent studies revealed additional targets and functions of piRNAs in various animals. piRNAs are ubiquitously expressed in somatic tissues of the pearl oyster Pinctada fucata , however, the role of somatic piRNAs has not well characterized. This study reports the PIWI/p...