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The role of estrogen receptor (ER) and its related effects is crucial for growth of breast cancer cells. This study aimed to assess the cytotoxic impact of alizarin in models of breast cancer in vivo and investigate its antiestrogenic and apoptotic properties. MTT assay was used to evaluate alizarin cytotoxic effect against MCF7 and MDA-MB-231. In vivo, 30 mice were insulted with Ehrlich tumor cells. They were randomly allocat...